I was using the simulator in cisTEM of alpha version (pre-compiled) with the tutorial written on the cisTEM-dev website: https://bhimes.github.io/cisTEM_docs/docs/sim/tutorials/tutorials.html
Section 3, calculate a stack of noisy particles, in that tutorial shows steps to simulate an isolated protein. I followed the instructions and I could reproduce Figures 1 A and B by modifying and executing bgal_flat.sh on my ubuntu 20.04. However, for Figure 1 C, after removing the flag, --save-detector-wavefunction, from optional_args in shell script (bgal_flat.sh), the generated image does not look identical with Figure 1 C which is very dark. In fact the generated image looks similar to Figure 1 B. I have no clue why this difference happened.
Could you help me with this situation? I attached the simulated image in png and my output messages shown on the terminal after runing the simulator.
Thanks for the useful debugging info and for testing out the simulator.
TLDR; you need to also have ctffind on your path to run the simulator. I should make that more clear in the docs, and have created a new issue to track this here.
For questions about alpha versions, please post over github using links from the developmental docs under the "Request new documentation or report bugs" banner on the landing page.
It looks like you are calling the binary you downloaded for the simulator directly, which is okay if you have another version of cisTEM somewhere on your path. ctffind is called to measure the "apparent" amplitude contrast, since this is generated by the atoms and not set explicitly by the CTF parameters. This way, the output star file has an amplitude contrast ratio that should match what would be fit by ctffind.
Thanks for your fast reply. I have copied ctffind to the folder to run the simulator, and I can see new results including ctf patterns on the power spectrum.
Besides, I have posted my simulation question to the github with your link, and am still waiting for answer.
This is the update about my low dose simulation issue.
I put cttfind together with the simulator and executed a simulation. But the simulator couldn't find ctffind as well.
So I went to modify the source codes, simulate.cpp and wave_function_propagator.cpp to make them find ctffind. The source codes were downloaded from https://github.com/bHimes/cisTEM_downstream_bah.git. Then I can reproduce a reasonable image result with the low dose simulation.
As for the high dose simulation, the simulated image shows the betagal and background are in white and black colors, respectivley. Those colors are inverted and different from the tutorial image, https://bhimes.github.io/cisTEM_docs/_images/Sim_tutorial2_1.svg
Why is that so?
On holiday, I'll get back to you early next week over on the github issues page. No need to duplicate your efforts over here : )
Cheers - Ben