Cannot reproduce the simulated image by using the simulator in cisTEM of alpha version

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Paul
Cannot reproduce the simulated image by using the simulator in cisTEM of alpha version

Dear all, 

I was using the simulator in cisTEM of alpha version (pre-compiled) with the tutorial written on the cisTEM-dev website: https://bhimes.github.io/cisTEM_docs/docs/sim/tutorials/tutorials.html

Section 3, calculate a stack of noisy particles, in that tutorial shows steps to simulate an isolated protein. I followed the instructions and I could reproduce Figures 1 A and B by modifying and executing bgal_flat.sh on my ubuntu 20.04. However, for Figure 1 C, after removing the flag, --save-detector-wavefunction, from optional_args in shell script (bgal_flat.sh), the generated image does not look identical with Figure 1 C which is very dark. In fact the generated image looks similar to Figure 1 B. I have no clue why this difference happened. 

Could you help me with this situation? I attached the simulated image in png and my output messages shown on the terminal after runing the simulator.

 

Best regards,

Paul 

 

 

 

himesb
Low dose simulation

Hi Paul,

 

Thanks for the useful debugging info and for testing out the simulator.

 

TLDR; you need to also have ctffind on your path to run the simulator.  I should make that more clear in the docs, and have created a new issue to track this here.

 

For questions about alpha versions, please post over github using links from the developmental docs under the "Request new documentation or report bugs" banner on the landing page.

 

HTH, Ben

 

Details:

 

It looks like you are calling the binary you downloaded for the simulator directly, which is okay if you have another version of cisTEM somewhere on your path. ctffind is called to measure the "apparent" amplitude contrast, since this is generated by the atoms and not set explicitly by the CTF parameters. This way, the output star file has an amplitude contrast ratio that should match what would be fit by ctffind.

Paul
Re: low dose simulation

Hi Ben, 

Thanks for your fast reply. I have copied ctffind to the folder to run the simulator, and I can see new results including ctf patterns on the power spectrum. 

Besides, I have posted my simulation question to the github with your link, and am still waiting for answer. 

 

Many thanks,

Paul

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