Hi everyone,
I am particle picking using RELION then using cisTEM for 2D class averaging. I import my files into cisTEM as a refine pkgs and everything works fine (initally). As I pick more particles in RELION, I import my updated file (with more particles) into cisTEM. However, I am running into an issues when class averaging. When I display my stack, I only see grey boxes (image attached below) and when I run class averaging all class averages are empty. Does anyone know what might be the cause and how I can fix it?
Many thanks,
Kasahun
Hi Kasahun,
When cisTEM imports a stack from relion, it doesn't change it. This implies there is already a problem with the stack. Can you display the stack in other programs?
Tim
Hi Tim,
I just opened the stack using EMAN. I see the same thing. I dont know how I can fix it.
Thanks,
Kasahun
Hi Kasahun,
I think this means your stack is broken. Can you try and regenerate it?
Tim
Hi Tim,
I just made another stack. I am getting the same thing.
Kasahun
How are you making the stack?
Tim