Class Averaging using refine pkgs from RELION

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kneselu
Class Averaging using refine pkgs from RELION

Hi everyone,

I am particle picking using RELION then using cisTEM for 2D class averaging. I import my files into cisTEM as a refine pkgs and everything works fine (initally). As I pick more particles in RELION, I import my updated file (with more particles) into cisTEM. However, I am running into an issues when class averaging. When I display my stack, I only see grey boxes (image attached below) and when I run class averaging all class averages are empty. Does anyone know what might be the cause and how I can fix it?

Many thanks,

Kasahun

timgrant
Hi Kasahun,

Hi Kasahun,

When cisTEM imports a stack from relion, it doesn't change it.  This implies there is already a problem with the stack.  Can you display the stack in other programs?

Tim

kneselu
Hi Tim,

Hi Tim,

I just opened the stack using EMAN. I see the same thing. I dont know how I can fix it.

 

Thanks,

 

Kasahun

 

timgrant
Hi Kasahun,

Hi Kasahun,

I think this means your stack is broken. Can you try and regenerate it?

Tim

kneselu
Hi Tim,

Hi Tim,

I just made another stack. I am getting the same thing.

Kasahun

timgrant
How are you making the stack?

How are you making the stack?

Tim

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