Hello,
I am encountering an issue of Refine Template after running Match Template not recording the alignment data into the database.
The output.mrc files reflect that refinement was performed (see attached).
I am using the distributed binary of cistem2-alpha, running refine_template on 1000 micrographs, and running 48-64 processes for these refinement jobs.
Any insight on what to do in order to get the match metadata written to the database/accessible?
Thanks for the help!
Hi,
unfortunately the Refine Template page in the GUI is not working. There are two options, you can either use the refine_template cli program or you can just do a manual refinement job using the template as a reference. (What you do depends a lot on what you are trying to get out of it.)
Johannes
Good to know.
I am trying to both refine initial hits in a template matching run against both the original template and an alternate template with an additional subunit then evaluate the SNR ratios.
When running refine_template from the command line, is there a straight-forward way to extract the alignment metadata and SNR scores from the .mrc outputs?
There is a program called make_template_result that takes the mrc outputs, finds the peaks, and spits out the results both on the commandline and to a xyz_coordinates file (that should also include angles and scores).
Clearly still a few rough edges, sorry.
No worries! I appreciate that it is still an alpha.
With this information, I can achieve what I have in mind.
Thank you for your prompt help!