Dear all
I recently started with cisTEM, I try to export a data set already refined from relion. I want to refine it with cisTEM. I understood from other discussions that I have to export the star file in particle stack. In this way I can’t export the metadatas for ctf correction in the star file as I wish to. Can you suggest me the best way to proceed in cisTEM for such situations?
Thank you by advance.
Julien
Hi Julien,
Which metadata do you want to import? per particle defocus values should be imported?
Currently, cisTEM will not import a star file from relion 3.0 without modification.
Tim
Hi Tim
Thank you for your response
Yes I thought about per particle defocus values that I previously refined in relion. I would like to CTF correct my particle stack.
Julien
Hi Julien,
If you have star file and a particle stack, in the relion 2.0 format. The star file should contain the defocus values, and they will be imported when you import in cisTEM.
Tim
Hi Tim,
Thank you, I'll make it at the relion2 format.
Best
Julien