I am new to cisTEM and recently collected dose-fractionation tilts at Janelia. When I added Directory to import movies for alignment, some tilts showed big shifts and blurry but when I just imported that blurry tilt only, and ran the same parameters, it turned out fine... Does that mean I should do one by one for tilt series?
For my image condition: 1) 2.93 e/A2 and 5 frames, because we would like to keep min dose for each frame to give density; 2) we did 3 degree increment from -60 to 60; 3) pixel size is 1.3 at super-res mode and binned by 2, 2.6A/pixel. 4) applied gain reference when importing.
Thanks for this amazing software!