I have two questions about conversion of relion data to cisTEM.
1. I would like to import a large Relion particle stack into cisTEM, but when I am trying to run relion_stack_create, I am running out of RAM (the number of particles is too big). What would be the best strategy or a tool to split and combine a large number of particles and to combine the star files? Does anyone have an efficient walkaround script for it?
2. Does it make sense to always --apply_rounded_offsets_only if the stack already has been refined? Would it computationally help cisTEM with the centering or should make no big difference? I guess if this option is not used, it would be easier to come back to the shifts and angles Relion produced for further comparison, wouldn't it?