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Import particle stack and metadatas from relion

julien

Import particle stack and metadatas from relion

Dear all
I recently started with cisTEM, I try to export a data set already refined from relion. I want to refine it with cisTEM. I understood from other discussions that I have to export the star file in particle stack. In this way I can’t export the metadatas for ctf correction in the star file as I wish to. Can you suggest me the best way to proceed in cisTEM for such situations?
Thank you by advance.

Julien

Tue, 11/26/2019 - 15:33

timgrant

Hi Julien,

Which metadata do you want to import?  per particle defocus values should be imported?

Currently, cisTEM will not import a star file from relion 3.0 without modification.

Tim

Tue, 11/26/2019 - 16:00

julien

Hi Tim

Thank you for your response
Yes I thought about per particle defocus values that I previously refined in relion. I would like to CTF correct my particle stack.

Julien

Tue, 11/26/2019 - 16:06

timgrant

Hi Julien,

If you have star file and a particle stack, in the relion 2.0 format. The star file should contain the defocus values, and they will be imported when you import in cisTEM.

Tim

Tue, 11/26/2019 - 16:09

julien

Hi Tim,

Thank you, I'll make it at the relion2 format.

Best

Julien