I exported an asset in relion format from cisTEM and it created a stack file and a star file. Can someone tell step by step how I can use these two files to run 2D classification in Relion? I tried, but it did not work for me. What I did in Relion, is as follows:
1. Imported motion corrected mrc files, that created micrographs.star file
2. used particle extraction as attached screenshot
where to use the stack file?
Thanks for your time,