Error message during AutoRefine

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rasmuskf
Error message during AutoRefine

Dear cisTEAM,

During an AutoRefine run in iteration 5 out of 20 I got an error message after the "calculating reconstruction step" saying,

error: mode 32618 MRC files currently not supported. 

The GUI then immediately aborted. When trying to open the already calculated volumes in chimera I get an error message saying that "file size is too small for grid size". Any suggestion if the already calculated volume can be used? Or should I retry the AutoRefine run?

Best,

Rasmus

timgrant
Hi Rasmus, 

Hi Rasmus, 

This sounds like something went wrong and the volume was not correctly written (is it possible you ran out of disk space in the scratch directory?)

I think you will have to retry the run - sorry!

Tim

rasmuskf
Hi Tim,

Hi Tim,

Disk space shouldn't be a problem. But out of curiosity, is there a way to see cisTEM's memory requirements for 3D refinements/classifications? The box sizes I use for my particles are in the 512-700 pixel range (1.1 Å/pix) and especially when I ask for multiple 3D classes it happens to me that the GUI suddenly aborts without providing any error messages.

Best,

Rasmus

timgrant
Hi Rasmus,

Hi Rasmus,

I think the memory usage should be less than 4 * the 3D size at most PER process.  So for 700 pixels, it would be ~5.2GB per process.

Tim

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