Hi, I am very new in Cryo-EM area.
Recently, I have collected Cryo-EM dataset with around 2,000 images. Every image contains 50-100 particles.
Then, I used cisTEM to do the auto-picking of the particles and directly went to 2D classification. From the 2D results, I picked up the good classes and will do ab-initio.
However, at this moment, my colleagues told me that I should manually clean the particles on every image after auto picking. They said that it will affect the final resolution if I don't do this cleaning step.
It will take me several days to clean particles on the 2,000 images, and you can imagine that this work is really boring.
So I would like to ask you, in your case, do you do manually particle cleaning before 2D classification?