Sorry for the stupid questions...after I used unblur from cisTEM to align the frames for each tomography tilt, the density values seem too small between -2 to 2 with Mean density at 0.94703 e-9. Briefly, I applied gain reference while I imported each set of frames, and bin 2 as suggested, other value in expert mode I kept as default. I am wondering: 1) whether the scaling is not correct? 2) How can I check scaling? 3) Is there a way to change the output mode to integer?