After determing the 3D map by cisTEM (Thank you for the fantastic UI and performance), I realized the pixel size provided from microscope is slightly larger than it should be.
Now I have calibrated pixel size and wonder what is the best way to recalculate the 3D EM map.
I could redo everything from movie alignment with new pixel size though I am looking for options to calibrate pixel resolution during refinement or creating refinement package.
Can cisTEM handle pixel size calibration instead of re-run everything? Thank you.