I am curretrly processing a particle stack extracted from relion with cisTEM 1.0.
I first did an auto refine that gave me a final resolution of 2.92A.
I then wanted to make a CTF refinment usinf the cistem "manual refine" option. I used pretty much the same parameters as the auto-refine did, only with the additon of the CTF refinement but I can't get a resolution higher than 3.94A with this method. I tried several parameters and it was always the same.
I may by wrong somewhere in the process, but I can't figure it out.
Do you have any idea how I could resolve this ?