I have been trying to get 2D classes for my data set using cisTEM but every time I run the classification job I only get gray boxes (see attached image). I used a small subset of my data (~30 images) and was able to get reasonable class averages so I know the parameters I am using work. My collaborator has also worked with the same data set in cisTEM and was able to get good 2D averages so I don’t suspect problems with my data set. I have had several instances where cisTEM has closed unexpectedly due to power problems with our workstation and/or the VNC viewer. When opening cisTEM and returning to a project I receive the following message:
“Each database should only be opened by one instance of cisTEM at a time, otherwise corruption is possible. If it exists, you should close the other instance before continuing, if this message is the result of a crash etc. then you can override and continue.
Do you want to overide?”
I selected “override” so that I could open the project to continue working but now I am worried this has caused a file corruption that is causing the problems with the 2D classification. Is that possible? Any insight into why I am not getting reasonable 2D averages and getting these gray images instead is much appreciated. Thank you.