Slave has disconnected errors

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Daniel Asarnow
Slave has disconnected errors

I am trying to troubleshoot a problem on our cluster where I get the message "Error: A slave has disconnected before all jobs are finished" in the GUI. Usually, this happens at the end of particle picking, or after the reconstruction phases of a multi-class refinement. There is one such message per running process.

I don't believe it's a cisTEM bug, but I was hoping you could shed some light on situations in which the slaves disconnect or exit early so I can figure out how to fix the cluster configuration (e.g. is there a timeout on IO? Can I increase output verbosity?). We have many compute nodes connecting to the same RAID/NFS server via 10G ethernet, but the interactive node that runs the GUI only has 1G network.

 

Best,

-da

kuenphonwu
We also have same issues of

We also have same issues of slave disconnection happened few times.  The "disconnect job" issue always happened at end of 2D classfication or Ab initio 3D model process.  When the error message pops up, the calculation kind of paused forever.  We ran cisTEM on a local GPU workstation (16 Xeon cores, 128 GB DDR4 ram, 25TB local HDD, 1080 Ti cards x4).

 

Best,

Kuen

Daniel Asarnow
Hi Kuen,

Hi Kuen,

Do you use default run configuration (17 processes in your case)? Also, how are your hard drives configured (how many drives, RAID level, filesystem type)? What did top/htop show during while the job was hung?

So far I only get this error when I use a certain network filesystem, so I strongly suspect a connection to IO availability.

kuenphonwu
Hi Daniel,

Hi Daniel,

We routinely ran 33 processes (16 cores, 32 threads). The HDD is configured by RAID 0 (10TB x2, 4TB x4, Hitachi enterprise SAS HDD), filesystem is ZFS.

When seeing the slave disconnection error, one CPU in htop was idling ( I usually got 1 slave disconnected). The werid thing is we reran same process without changing settings, it often finished normally (sometimes need to rerun 2 times). I am sure no other disc I/O process except cisTEM during calcuation. 

giuliazanetti
Dear Tim and cisTEM users,

Dear Tim and cisTEM users,

I run into similar issues with my (first) 2d classification job.

It goes through to round5.

If I run it from the cluster, I get a series of Error: A slave has disconnected before all jobs are finished. It then remains idle and the job never progresses past round 5.

If I run it interactively from a multicore workstation I get no error at all, but still the job gets stuck at round 5 and never goes forward.

In both cases if I look at the results I notice that for some rounds the classes are nonsensical: either totally grey, or weird pixelated boxes. Still, round 1,2 and 4 show what expected.

 

My first guess was that there must be some corrupted particles that when picked up in the randomly selected subset cause trouble, but I manually visualised all of them and couldn't see anything obvious. I got no warning or anything while extracting them.

 

Not quite sure where to start from to troubleshoot, any help would be much appreciated.

 

Thanks

Giulia

timgrant
Hi Giulia,

Hi Giulia,

Does it reproducibly stop at round 5?  If so, would be able to share the stack with me, so that I can look into it?

Cheers!

Tim

giuliazanetti
Hi Tim,

Hi Tim,

It does reproducibly get stuck at round5.

This is a groel test dataset that was collected on our new microscope for a test. I was handed the pre-motioncorrected sums and pre-picked .box files.
I imported DW and noDW, run ctffind through cisTEM (on noDW), then converted .box to .txt with a script I found on the forum and imported that too.
Then I followed the tutorial to extract particles (from DW), and run 2D class.

(FYI same .box and .mrc files imported in relion run just fine through the end)

What is the best way to share the stack with you?

Thanks a lot!!!

Giulia

timgrant
Hi Giulia,

Hi Giulia,

If you can export your refinement package - you will get a stack file and a .par file,  how big is that?

Do you have a server I can connect to, to download it?

Cheers,

Tim

wxh180
Hi Tim,

Hi Tim,

I've come across the same problem. The refinement package is working for auto-refine with 1 class. When I create another refinement package for 3 classes, I came across this error during auto-refinement. Could you let me know how to debug this problem? Thanks!

 

Best,

Wei

timgrant
Hi Wei,

Hi Wei,

You are getting " Error: A slave has disconnected before all jobs are finished" printed in the GUI during an autorefine with 3 classes.  Does it always happen at the same stage, if so - at what stage?

Thanks,

Tim

rdrighetto
Hi,

Hi,

I just want to report that I was also getting "Error: A slave has disconnected before all jobs are finished" errors and the jobs would hang indefinitely during Movie Alignment. This would happen both when submitting jobs to SLURM or when running locally on a computing node ("Default local" profile). This was strange because normally cisTEM runs fine on our cluster. Turns out for this particular dataset I was using the option "Include All Frames in Sum?" to select only a subset of the aligned frames. When disabling this option (i.e. including all frames, which is default), the job ran normally to completion.

I'm not sure how using this option would cause such an error but this could be a useful hint for debugging.

Cheers,

Ricardo

timgrant
Hi Ricardo,

Hi Ricardo,

Thanks for the info - i'm sure it will be helpful!

Tim

jamonshi
I ran into exactly same issue

I ran into exactly same issue as rdrighetto mentioned. When I only select subset of frames for movie alignment, I got multiple "Error: A slave has disconnected before all jobs are finished". Everything is OK when I include all the frames.

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