Reading Tiff format in cisTEM

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phonchi
Reading Tiff format in cisTEM

Dear developers:

I have some movie files store in mrc format. I would like to reduce the size so I convert them into tif format using 

mrc2tif –s –c lzw movie_file.mrc movie_file.tif

However, after conversion, I can not align them using cisTEM.

The error is as follows:

============================================================================

Error. Unsupported sample format: 2. Filename = /home/users/phonchi/appo_10/test.tif, Directory # 0

Error. Unsupported sample format: 2. Filename = /home/users/phonchi/appo_10/test.tif, Directory # 1

Error. Unsupported sample format: 2. Filename = /home/users/phonchi/appo_10/test.tif, Directory # 2

Error. Unsupported sample format: 2. Filename = /home/users/phonchi/appo_10/test.tif, Directory # 3

Error. Unsupported sample format: 2. Filename = /home/users/phonchi/appo_10/test.tif, Directory # 4

Error. Unsupported sample format: 2. Filename = /home/users/phonchi/appo_10/test.tif, Directory # 5

....

============================================================================

 

Any suggestions? Thanks in advance!!

timgrant
Hi,

Hi,

This means that the tif format cannot be read by the released version of cisTEM.  For example, 4-bit files cannot be read by the released version (although it will be readable in the next release).  Are these files 4-bit?

Cheers,

Tim

phonchi
Thanks for the reply! How do

Thanks for the reply! How do I check whether my mrcs or tiff is 4-bit files or not? If it is 4-bit files, does it mean that currently, I should not convert it into tiff files?

Thanks in advance!

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